nanoLC systems for speed, sensitivity, resolution, and reliability

Eksigent’s second-generation NanoLC™ system delivers stable, reproducible separations for peptide/protein identification, phosphopeptide analysis, biomarker discovery, metabolomics, and more.

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nanoLC-1Dplus product note

The NanoLC-1Dplus is a one-dimensional nano HPLC configured with a third pump, and the NanoLC-2D is a two-dimensional nano HPLC that lets you set-up a 2D method in seconds. Both systems come with an optional autosampler for rapid injection of large sample volumes supporting full loop, partial loop, and zero sample loss modes. The systems easily integrates with Thermo Electron’s Xcalibur® software, Bruker Daltonics’ HyStar® system and ABI/Sciex Analyst® software.

NanoLC-1Dplus system for speed, sensitivity, resolution, and reliability

The NanoLC-1Dplus system, a compact proteomics lab workhorse, uses Microfluidic Flow Control (MFC) to enable automated peak parking for extended MS/MS analysis of analytes of interest. The Eksigent NanoLC-1Dplus system comes with a dedicated sample-loading pump for fast, automated trapping of high-volume proteomics samples.

Ideal for proteomics and biomarker discovery applications, the NanoLC-1Dplus system allows sample loading at higher flow rates for significant timesavings and flexibility. The system loads large samples as rapidly as 30 uL/min using a dedicated sample-loading pump. The system also features pump and flow controller technology that allows precise operation at pressures up to 5,500 psi.

If you’re looking for reproducible nanoscale HPLC performance, you’re looking in the right place. The NanoLC-1Dplus system offers speed, sensitivity, and resolution—along with a simple, reliable architecture that precisely controls flow rate down to 20 nL/minute without flow splitting.

Identify more proteins from complex mixtures with automated 2-D chromatography

The NanoLC-2D system provides all of the performance capabilities of the NanoLC-1Dplus with the added capability of fully automated 2-dimensional separations. The result is high sensitivity, fast column format changes, low-solvent waste, and highly reproducible separations.

The NanoLC-2D system is composed of two complete binary-gradient nanoflow chromatography systems that can be configured for automated two-dimensional separations or as parallel, independent binary gradient HPLC systems. With its revolutionary pumping system, you can operate in three modes: 1D, 2D, or parallel separations.

NanoLC system features-at-a-glance

Optimized for ultra-low flow rates of first dimension 1-20 µL/min, second dimension 50-1000 nL/min.
Novel pumping system eliminates the need for flow splitting, providing excellent run-to-run reproducibility.
Use automated peak parking for extended MS/MS analysis to improve peptide sequence coverage.
Take advantage of nanospray sensitivity.
Active flow control of each mobile phase ensures precise gradient delivery.
Quickly responds to changes in flow rate set point in less than two seconds.
Reduce solvent consumption by 99%!

nanoLC-1Dplus system specifications

system components

Binary gradient pump
Dedicated sample loading pump
10-port, low-volume, biocompatible injection valve (Ti or PAEK)
Optional temperature-controlled autosampler (96- or 384-well plate compatible) with a 6-port biocompatible sample injection valve (Ti or PAEK)

flow rate range
20-1000 nL/min (other ranges available with flow module swap) 1-30 µL/min for sample loading pump

pump type
Microfluidic direct pumping system with independent flow control feedback for each mobile phase. Retention time RSD < 0.5% at 500 nL/min

gradient formation
High-pressure gradient mixing, no flow splitting

delay volume
65 nL from mixer to column

delay time
< 25 sec at 500 nL/min

mobile phase compatibility
All mobile phases compatible with 316 stainless steel, PEEK, and silica

injections
Standard, metered and rapid injection modes

injection volume
200 nL - 50 µL; option for larger sample volumes available

columns
Optimized for use with 50-150 µm i.d. columns, 5-15 cm in length

peak parking
Response time from 500-100 nL/min typically < 4 seconds at spray tip. Response time can vary with external hardware configuration

autosampler
One 96- or 384-well plate capacity Peltier type temperature maintained sample compartment. 5-40° C; cooling capacity 12° C below ambient Dual needle design for seal piercing Integral 6-port biocompatible injection valve

system control
Computer with graphical user interface for control of all system parameters. Software allows import of run tables and creates CDF, text, and Excel files for data export and analysis. Tracking of instrument runtime, column usage, total injections, solvent usage, and error codes. System drivers available for Thermo Electron’s Xcalibur™, Applied Biosystems/MDS SCIEX Analyst®, and Bruker Daltonics’ HyStar™ mass spectrometer software.

power requirements
100-240 VAC, 4/63 Hz, 3A

dimensions

Width: 14 inches (35 cm)
Depth: 15 inches (38 cm)
Height: 8 inches (20 cm)
Computer: additional lab spaced needed for computer, keyboard, mouse, and monitor

nanoLC-2D system specifications

system components

2 independent binary gradient pump systems
10-port column switching valve, accommodating 2 trap columns and an analytical column
Integrated temperature-controlled autosampler (96- or 384-well plate compatible) with a 6-port biocompatible sample injection valve

flow rate range

First dimension: 1-20 µL/min
Second dimension: 20-1000 nL/min

pump type
Microfluidic direct pumping system with independent flow control feedback for each mobile phase Retention time RSD < 0.5% at 500 nL/min.

gradient formation
High-pressure gradient mixing; no flow splitting

delay volume

First dimension: 300 nL from mixer to column
Low-flow dimension: 65 nL from mixer to column