Maximum pressure of 10,000 psi
The entire system is designed to operate at pressures up to 10,000 psi. This allows the use of columns with small diameter stationary phase particles (1.5 to 3 µm) delivering fast, high resolution separations (Figure 1).
With MFC technology, direct pumping is used to deliver accurate, repeatable gradients. Each pressure source delivers fluid with the actual flow rate monitored using the flow modules in each channel. A feedback loop makes real-time adjustments to the flow rate.
Precise metered injections
Precise and accurate pump flow control, in combination with a high speed injection valve, allow for programmable metered injections with volumes as low as 100 nL.
The filled injection loop is switched to the inject position for a pre-determined length of time to displace the programmed amount of sample. This feature can be extremely useful in cases where the upper dynamic range of a linear calibration curve is insufficient to cover samples of unexpected high concentration. Instead of diluting the entire series of samples and re-analyzing them, one can simply re-inject the same samples using a smaller injection volume.
As illustrated in Figure 2, by reducing the injected volume from 5 µL to 0.5 µL, the upper linear dynamic range is effectively extended by an order of magnitude. There is no need for additional sample dilution steps or exchange of the injection loop.
Extreme reliability
Compared to a conventional reciprocating pump with an electric motor, Eksigent's pneumatically driven pump design reduces the number of moving parts significantly. The end result is an extremely reliable pump requiring minimal preventive maintenance. The same pump design has been a key component of Eksigent's widely adopted line of NanoLC systems that have been in successful operation since 2004.
Easy operation
With Eksigent's Control Software, users can rapidly create or modify separation methods. An intuitive interface makes the system easy to learn, and the specialized diagnostics tools make it easy to operate and maintain. The software continuously monitors pressure and flow rate, and provides real-time views of gradient composition and column backpressure.
Green HPLC
Analyses using 1 mm ID columns use flow rates from 50-150 µL/min. With typical run times as short as 60 seconds, this means less than 150 µL of solvent is consumed per analysis. Compared to a conventional system running at 1 mL/min for 5 minutes using 4.6 mm ID columns, the ExpressHT-Ultra saves up to 95% of the solvent.
In addition to reduced solvent acquisition/disposition costs, lower flow rates and lower solvent consumption will decrease the burden on your mass spectrometer source and vacuum system, potentially reducing downtime and environmental emissions.
Save space
The ExpressHT-Ultra's compact design takes less than 25 inches of bench space. Standing less than 30 inches tall, the entire system easily fits on a laboratory cart for easy mobility.
Read about the new system's integrated system configurations and specifications, download the expressHT-Ultra product note, or watch a technology animation.
