Rapid HPLC provides high throughput profiling of compound lipophilicity.
Determination of the physicochemical and ADMET properties of compounds early in the drug discovery process can help prioritize leads from high-throughput screening and reduce the failure rate of drug candidates during development. One such profiling assay is compound lipophilicity, which is typically measured as octanol/water partitioning, or log P. This property is an important parameter for predicting oral absorption. An HPLC-based method for modeling octanol/water partitioning has been developed, called the chromatographic hydrophobicity index (CHI).
The Chromatographic Hydrophobicity Index method has been successfully transferred from conventional HPLC format to the ExpressLC system. The ExpressLC system shortens the sample run from 6 minutes to 1.5 minutes, without a loss in performance. Generated CHI values show close agreement between the two systems. The ExpressLC system requires less than 1% sample than the conventional system per injection. Retention time precision over the course of 10 consecutive runs in 15 minutes shows excellent reproducibility, with the highest recorded RSD of 0.18% for the first peak. CHI sample throughput on the Express-100 single-channel system will exceed 40 samples per hour. On the Express-800 system throughput will exceed 320 samples per hour.
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